首页>
外国专利>
Human embryonic stem cells genetically modified to contain a nucleic acid and cultured with fibroblast growth factor
Human embryonic stem cells genetically modified to contain a nucleic acid and cultured with fibroblast growth factor
展开▼
机译:经过基因修饰的人类胚胎干细胞含有核酸并与成纤维细胞生长因子一起培养
展开▼
页面导航
摘要
著录项
相似文献
摘要
This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.
展开▼