Cloning of Tumor Suppressor Genes in Prostate Cancer by a Novel Tumor Reversion Method.

摘要

We have developed a novel approach to the cloning of tumor suppressor genes in prostate cancer. We have transferred large pieces of human DNA, cloned into bacterial artificial chromosomes (BACs), into human prostate cancer cell lines. We then tested the ability of the transferred human DNA to revert (render less tumorigenic) the neoplastic phenotype of the cancer cell lines, using several criteria, including morphological changes in the cells, doubling time and growth in soft agar. We have found several BACs that revert human prostate cancer cell lines in this assay. We are now testing the ability of cDNAs encoded by genes on these BACs to revert the cell lines, using identical assays. This process should allow us to identify the putative tumor suppressor gene on the BACs. Our approach is potentially applicable to the cloning of any human prostate tumor suppressor gene, and thus is of potentially major importance.