Novel Peptide/Protein Delivery System Targeting erbB2-Overexpressing Breast Cancer Cells

摘要

The major goal of this study is to develop a penetratin-based delivery system targeting specifically ErbB2-overexpressing breast cancer cells. During this funding year, we focused on the delivery of erbB2 signal-blocking ESP peptides (objective 2) . Because of the complexity of biotin-penetratin- AHNP-ESP, the synthesis was unsuccessful. We therefore began to try different approaches. We take Stat3 as an alternative target because we found that Stat3 activation is correlated with erbB2 expression levels, which constitutes a promising therapeutic target in treating erbB2-overexpressing breast cancers. We successfully synthesized Stat3-blocking peptide (Stat3BP), and further conjugated Stat3BP to NeutrAvidin. Through the interaction between NeutrAvidin and biotin, Stat3BP could be linked to biotin-penetratin-AHNP.