Optical Imaging of Mammaglobin Expression of Breast Cancer

摘要

Mammaglobin (MMG) is a breast-specific glycoprotein that is over- expressed in 80% of all primary and metastatic breast cancer. Despite its uniqueness as a breast-specific cancer marker, the utility of MMG in breast cancer imaging has not been explored. Consequently, the goal of this project is to evaluate the potential of imaging the expression of MMG in established human breast cancer models. TO accomplish this goal, we labeled polyclonal and monoclonal anti-MMG antibodies with a near infrared fluorescent probe for optical imaging and 64Cu-DOTA for positron emission tomography (mPET). Preliminary results indicate that the mPET imaging with 64Cu-DOTA-anti MMG monoclonal antibodies showed predominant liver uptake in mice. In contrast, the fluorescent-labeled derivative of polyclonal MMG antibody primarily accumulated in the tumor, kidneys and liver. We have also developed a tumor model with increased expression of MMG. Our preliminary results appear to support the hypothesis that targeting the expression of MMG will enhance the localization of breast cancer tumors.