Inhibitors of Apoptosis Affect DNA Degradation and Repair in Sulfur Mustard (HD)-Exposed Human Epidermal Keratinocytes (HEK); Conference paper

摘要

In cultured HEK, we observed that HD (0.3-1.0 mM)-induced DNA damage triggers DNA repair and apoptosis, which may be interdependent. We studied the effects of a general caspase inhibitor, Z-VAD fmk (0.004 mM) and the Fas (CD95) receptor (induces apoptosis on Fas ligand binding) antibody on DNA damage and its repair in HD-exposed HEK. Both Z-VAD-fmk and the CD95 antibody reduced HD- induced DNA degradation possibly by decreasing DNA degradation or enhancing DNA repair or both. These inhibitors may be useful for modulating DNA repair and apoptosis in HD-exposed cells with potential applications in medical management of HD-induced vesication.