Investigating the Role of FIP200 in Mammary Carcinogenesis Using a Transgenic Mouse Model

摘要

In this report we give a preliminary account on the consequences of mammary-specific Focal Adhesion Kinase (FAK) deletion in mammary-specific polyoma middle-T transgenic mice. We monitored mammary carcinogenesis in positive control (FAKFlox/Flox; MMTV-PyVT) and target (FAKFlox/Flox; MMTV-Cre; MMTV-PyVT) females. We found that mammary-specific FAK deletion lengthens the tumor-free interval by 33.2 days. We also found that cumulative subcutaneous mammary tumor burden and tumor growth rate were larger in positive control females than in target females. Additionally we also found that FAK is virtually not expressed in mammary tumors from target animals but FAK expression was abundant in positive control animals. Transgenic polyoma middle- T antigen expression was similar in mammary tumors from both positive control and target females. Based upon the preliminary data we conclude that FAK although not absolutely required but it still plays a contributory role in polyoma middle-T antigen induced mammary carcinogenesis in female mice.