Utilization of Military-Relevant Muscle Injury Models to Identify Pharmacological Treatment Strategies

摘要

This study was designed to characterize critical molecules involved in skeletal muscle response to ischemia-reperfusion (I/R) and blunt trauma injury in order to identify strategies for pharmacological intervention. For I/R injury, a tourniquet was applied to rat hind limbs for 3 h. Extensor digitorum longus (EDL) muscles from the healthy and I/R leg were harvested 2 h post-reperfusion for analysis. For the blunt trauma model, mice were anesthetized, the tibialis anterior (TA) muscle was exposed, and injury was induced by applying a steel probe (cooled to -20 C) to the belly of the TA muscle. Muscle samples were collected from the healthy and injured leg 3, 10, 24, 48, and 72 h post-injury. Quantitative Real Time Polymerase Chain Reaction (qRT-PCR), immunoblotting, and immunohistochemistry were used to quantify/localize analytes of interest. I/R resulted in a significant.