Modification of the Method of Riley, Proemmel and Franke for Determination of Oxygen and Carbon Dioxide Tensions in Blood.

摘要

A method for the direct estimation of blood gas tensions (Po2 and Pco2) was described by Riley et al. in 1945 (1). In essence the method required that a small bubble of alveolar gas be equilibrated with 1 ml of blood at 37deg C in each of two Roughton-Scholander (R-S) syringe analyzers (2). The gas bubbles were then analyzed for carbon dioxide and oxygen, and the blood Po2 and Pco2 estimated from the means of the duplicate analyses. Various modifications of this technique have been introduced in the last 9 years, and it is the purpose of this paper to describe the changes which have been adopted in this laboratory. An evaluation of the results obtained with the present technique is included. JMD.