A THEORY AND METHOD FOR THE SPREADING OF PROTEIN MONOLAYERS

摘要

Protein monolayers, or true H-films, can be spread quickly and without loss on distilled water or dilute buffer solutions, if the protein solution before reaching the water surface of the film trough is spread out into a very thin layer around a glass rod. During the flow time along the glass rod the vast majority of all protein molecules diffuse to the water/air interface and spread, if flow rate, protein concentration, glass rod length, and diameter are chosen correctly. The influence of these parameters is investigated theoretically, and the theoretical predictions are compared with results from spreading experiments.