Electroimmunodiffusion Studies of alpha Chain, Secretory Piece and Secretory IgA.

摘要

Commercially available antisera to secretory piece, colostrum, and alpha chain were evaluated for use in electroimmunodiffusion of secretory IgA and secretory piece in biological fluids. Quantitation of secretory IgA presents difficulty since antisera to alpha chain indiscriminately precipitates both serum IgA and secretory IgA (11S) and production of antisera specific for antigenic determinants on secretory IgA is laborious and expensive. Problems in using currently available commercial antisera to colostrum and free secretory piece arise because they are multispecific. This report describes a technique modification to overcome that difficulty. Although monospecific antisera to I or P determinant would differentiate secretory piece or secretory IgA with one rocket precipitate, that same precipitate is identifiable in a multi-precipitate pattern by using a pure reference standard for establishment of a line of identity. The high level sensitivity of EID allows quantative assay of proteins in biological fluids collected in microliter amounts.