The Demonstration of Alternate Complement Pathway Activity by 'Fusobacterium polymorphum' in the Presence of Ethylene Glycol tetraacetic Acid.

摘要

A complement consumption assay was used to investigate alternate pathway activation by Fusobacterium polymorphum in quinea pig sera treated with the divalent cation chelators, EDTA and EGTA. In the presence of 0.001 M EGTA, a cell wall preparation of F. polymorphum and inulin reduced the hemolytic complement activity to the same degree as that observed in the non-chelated serum controls. A 0.001 M concentration of EDTA inhibited the anticomplementary activity of F. polymorphum, inulin, and the hemolysis of EA. It was also shown that a concentration of 0.001 M Mg++ reversed the inhibitory effect of EDTA on the classical pathway EA system and the anticomplementary activity displayed by F. polymorohum cell walls and inulin. The addition of the same concentration of Mg++ to the eDTA tests did not augment the consumption of complement via the alternate pathway, nor did it reverse the inhibition of the classical pathway.