Two Novel Methods for the Preparation of Erythrocyte Stroma Free from Haemoglobin Compared to the Conventional Centrifugation Technique

摘要

The removal of haemoglobin from haemolysed erythrocyte stroma by washing over a membrane filter or by passage through a column of porous gel was compared to the removal achieved by washing in the centrifuge. The column method was preferable for an initial rapid clean-up, which could be followed by a further pass through the column, or by centrifugation to concentrate the suspension. Membrane filtration was useful when total recovery of a small sample of storma was required.