Search for Hepatitis Viruses by New Methods.

摘要

Consistent antibody activity was noted in mice immunized with P22, but the immunogenicity was markedly lower than that noted in mice inoculated with the intact particle vaccine. Inoculation of P22 vaccine in conjunction with tetanus toxoid significantly increased the immunogenicity of the P22 component when saline suspensions were compared. Recent studies have shown that patients who have recovered from HBV infections develop a specific cellular cytotoxicity toward the PLC/PRF/5 hepatoma cell line. To facilitate testing of large numbers of mouse antisera, a micro solid-phase radioimmunometric assay (micro-SPRIA) and an enzyme-linked immunosorbent assay (ELISA) were developed and compared to the commercial radioimmunoassay (Ausab) for detection of anti-HBs antibody. Preliminary data were presented in conjunction with an undeantured P22-P25 polypeptide vaccine prepared by the technology of Zuckerman and coworkers. the polypeptide material, isolated in micelle form, retained high levels of HBsAg activity when tested by solid-phase radioimmunoassay in contrast to the low activity observed with SDS-denatured material.