Preparation and Characterization of Mouse and Human Monoclonal Antibodies to Botulinum Toxins.

摘要

The goal during this year of the project was to begin preparing and characterizing mouse and human monoclonal antibodies to botulinum toxoids. The initial step was to refine enzyme immunoassays for identifying both mouse and human monoclonal antibodies in hybridoma culture supernatants. Progress in this area has been good, though minor technical problems remain to be solved. Optimization of these assays was accomplished with hyperimmune mouse and human antisera obtained from Dr. Martin Crumrine, USAMRIID. Keywords: Mice, Human monoclonal antibodies, Enzyme immunoassays, Type B toxoids, Type E toxoids, Toxic, Microbiology, Medical research, Botulinum toxins. (jg)