Clonal Analysis of HLA-DPw1 (SB1) Associated Allodeterminants: Recognition of Novel Epitopes and Evidence for Quantitative Variation in Class II Antigen Expression

摘要

Alloreactive human T-lymphocyte clones were derived from the SBI (HLA-DPw1)-specific, primed lymphocyte typing cell line SB1A used in the Ninth International Histocompatibility Workshop. The clones from two separate subclonings were analyzed for their proliferative patterns in panel experiments with cells from unrelated individuals and in family segregation analyses. While only one clone gave a perfect correlation with the DPw1 specificity, the majority of clones recognized multiple specificities apparently associated with but not identical to HLA-DPw1. Most clones defined 'splits' or subset of DPw1 and some also displayed 'extra' reactions with DPw1-negative stimulator cells. Further evidence was also found that, of the molecules bearing epitopic subsets associated with DPw1, some may be selectively expressed on the cell surface whereas the surface density of other DPw1-associated antigens may be varied. Thus, the HLA-DP region appears to encode a complex array of alloantigens and is in this regard similar to the HLA-DR region.