Effect of Recombinant GM-CSF on the Recovery of Monkeys Transplanted with Autologous Bone Marrow

摘要

A variety of glycoproteins with hormone-like stimulatory effects on hematopoietic progenitor cells have been purified and characterized. In recent articles, the molecular and biochemical properties of specific murine and human glycoprotein, granulocyte-macrophage colony stimulating factor, were comprehensively reviewed. Recombinant human granulocyte macrophage-colony stimulating factor (rhGM-CSF) has been shown to stimulate the proliferation of granulocyte-macrophage colonies in vitro from the bone marrow (BM) of humans and primates. Recombinant human GM-CSF was also shown to stimulate eosinophil and multipotent progenitor cells and have burst-promoting activity. Mice and nonhuman primates have been used to evaluate the in vivo effects of recombinant GM-CSF. In a recent report, the continuous intravenous administration of rhGM-CSF, both before and after autologous bone marrow transplantation, led to an accelerated neutrophil and platelet recovery. In this report, the regulatory function of rhGM-CSF in hematopoietic regeneration is elucidated using an autologous bone marrow transplantation model with nonhuman primates. The protocol was designed to evaluate the regulatory effects of rhGM-CSF during early hematopoietic regeneration.