Norovirus Real Time RT-PCR Detection Technology Transition to the Joint Biological Identification and Diagnosis System (JBAIDS).

摘要

The fundamental objectives of this AFMSA FY10 O&M study was accomplished; real-time diarrheal disease causative agent detection capability. Highly sensitive and specific Norovirus GI and GII TaqMan assays were developed using the RAPID. The assays are applicable for use with diverse real-time PCR instrumentation to include the JBAIDS, HTS, and micro-array based systems. Project funding was provided by the Air Force Medical Support Agency (AFMSA), Research, Development and Innovations Directorate (SG9), Office of the Surgeon General (AF/SGR) Falls Church, Virginia and the Military Infectious Diseases Research Program (MIDRP), USAMRC, Fort Detrick, Frederick, Maryland. This project was conducted by the Enteric Diseases Department, Armed Forces Research Institute for the Medical Sciences (AFRIMS) and Clinical Research Division (CRD)/59th MDW. This project was jointly funded and executed under memorandum of agreement (MOA) between Walter Reed Army Institute of Research (WRAIR), Silver Spring, Maryland & 59th Medical Wing (MDW) Lackland AFB, Texas (MOA 2007 - 2012. Agreement No.: DODI 4000.19; AFI 25-201).