Purified Human and Recombinant Murine Interleukin-1 alpha Induced Accumulation of Inflammatory Peritoneal Neutrophils and Mononuclear Phagocytes: Possible Contributions to Antibacterial Resistance

摘要

The purpose of this project was to examine the influence of parenteral administration of interleukin-1, a cytokine with diverse biological activities, on antibacterial resistance in a laboratory rodent model. The first documented that intraperitoneal injection of minute quantities (0.1-1.0 micrograms per mouse) of interleukin-1 resulted in a rapid influx of inflammatory neutrophils. Neutrophil accumulation did not result from contamination of the interleukin-1 with bacterial lipopolysaccharide, nor was it abrogated by treatment with indomethacin, an inhibitor of prostaglandin synthesis. We also observed a small but significant increase in the number of inflammatory macrophages at last timepoints. We went on to show that prophylactic or concomitant administration of interleukin-1 (0.17 micrograms per mouse) significantly enhanced the resistance of recipient mice to a challenge infection with the facultative intracellular pathogen Listeria monocytogenes. Protection was not caused by contaminating bacterial lipopolysaccharide. Interleukin-1 mediated protection was associated with a rapid burst of serum colony--stimulating activity.