Activated Human Langerhans Cells Express mRNA for Interleukin-1(alpha) and Interleukin-1(beta) and Produce These Cytokines but do not Secrete Them.

摘要

Human Langerhans cells (LC) were isolated from epidermal cell preparations by panning with mouse anti-CD1 monoclonal antibody. RNA was prepared and probed for the presence of mRNAs for various cytokines using radiolabled cDNAs. After stimulation with phorbol myristate acetate LC express RNA for IL-1alpha and IL-1beta and produce proteins but do not secrete them at detectable levels. LC-associated Il-1, particularly IL-1alpha, may play a role in antigen presentation. PMA did not induce IL-6 expression in LC. The addition of lipopolysaccharide, a muramyl dipeptide analog, ionomycin, insulin-like growth factor 1 or IL-6 did not induce IL-1 mRNA in LC. Glucocorticoids did not detectably affect IL-1 alpha or IL-or IL-1 beta mRNA levels following PMA induction. Thus the inducers and regulators of IL-1 formation in human LC and monocytes are not identical. (js)