Effect of Red Blood Cell Transfusion on Acute Bacteremia in Dogs

摘要

Non-viable red blood cells are rapidly cleared from the peripheral blood by the reticuloendothelial system. Since bacteria present in the blood stream are also cleared by the reticuloendothelial system, the possibility that non-viable red blood cells would interfere with the clearance of bacteria has been raised. Groups of dogs were studied in whom an experimental bacteremia was produced by the injection of E. coli (1 x 10(exp 10)/kg). Bacterial clearance, endotoxin clearance, changes in mean arterial pressure, changes in oncotic pressure and coagulation responses were monitored before and following transfusion of 450 mls. of blood preserved with acid-citric dextrose or 450 mls. of heparinized blood stored without preservative. In the latter blood product the majority of the red blood cells were non-viable. Clearance of E. coil over a 4 hour period was similar in the two transfused groups and in a control group without blood transfusion. Mean arterial pressure and blood pH fell significantly in all groups, but was again not significantly different among the groups studied. Endotoxin clearance, changes in oncotic pressure, blood oncotic proteins, and parameters of the blood coagulation system were also similar in all three groups. The results of this study indicate that in normal dogs made acutely bacteremic with E. coil, there were no significant differences over a 4 hour period in their hemodynamic or hematologic responses following transfusion of viable or non-viable red blood cells.