Method for determinating dog blood transfusion compatibility and kit monoclonal antibodies and hybridoma cell-line for the same

摘要

The present invention relates to a method for determining the suitability of blood transfusion in dogs, and to kits, monoclonal antibodies and hybridoma cell lines therefor. An antigen-antibody reaction is performed on a blood sample of a dog for transfusion and a blood sample of a dog to be transfused, and the monoclonal antibody produced by hybridoma cells of Accession No. KCTC 13495BP and a hybridoma of Accession No. KCTC 13496BP It relates to a method for determining transfusion suitability of a dog, and a kit, a monoclonal antibody and a hybridoma cell line for contacting the monoclonal antibody produced by the cell with each sample and detecting antigen-antibody binding. The method of the present invention can effectively complement the problem of conventional dog transfusion suitability determination by using antigens of dog blood independent from the Dal and Shigeta system as well as the DEA blood type system, which is a typical representative dog transfusion suitability method. have. In particular, the monoclonal antibody of the present invention has high specificity for each antigen, thereby enabling very accurate and easy identification.