Technique for Repeated Collection of Cerebrospinal Fluid from Cisterna Magna ofAnesthetized Strain 13 Guinea Pigs

摘要

To study biochemical changes in cerebrospinal fluid (CSF), we developed areliable technique for repeated collection of CSF in anesthetized strain 13 guinea pigs. The animal's head was mounted in a stereotaxic instrument with ventral tilt at 30, and cisternal puncture was made with an L shaped, 23 gauge needle through the shaved skin. Clear CSF was collected in a 1 ml syringe surrounded by crushed ice. Each collection procedure lasted for 3 min, and three consecutive collections produced about 0.2 ml of CSF. Sampling was repeated at 3 hr intervals. With intravenous saline infusion (10 ml/kg.hr), a total volume of 0.6-1.0 ml of CSF was collected over 6 to 12 hr. Animals maintained a mean blood pressure, heart rate, and minute volume, with few changes during CSF sampling for the entire collection.