Identification of rice hybrids and their parental lines based on seed, seedling characters, chemical tests and gel electrophoresis of total soluble seed proteins.

摘要

Identification of rice genotypes is of prime importance to ensure quality seed, which is required for achieving global food demand. Three rice hybrids and their parental lines were identified on the basis of seed colour, seed size and 1000 seed weight, response to different chemicals (Phenol, Modified phenol with Copper sulphate or Ferrous sulphate, NaOH, GA3 and 2,4-D) and electrophoresis of soluble seed proteins (SDS-PAGE). All the genotypes were identified by using key, based on seed colour, 1000 seed weight and seed size except for the female lines and their maintainers. No individual chemical test was able to distinguish all the genotypes. However, the combinations of different rapid chemical tests were useful in identification of individual genotypes. While, all the cultivars were identified successfully by using electrophoresis of seed protein (SDS-PAGE) and hence, it could be used as a powerful tool to identify every genotype in a short period of time.