可溶性寡糖和小分子的热激蛋白与杂交水稻种子成熟过程中发芽能力及种子活力相关

摘要

To study the relationship between seed soluble oligosaccharide, small heat shock protein (sHSPs) and seed vigor, we systematically studied their changes during hybrid rice seed maturity. The mean fuzzy subordinate function values of germination percentage, germination energy, germination index, vigor index and mean germination time, root number, root length, shoot length and seedling weight were analyzed. The results indicated that seeds harvesting at 19 to 34 days after pollination (DAP) had a higher viability and vigor. The seed moisture content had a decline trend, with a decrease from 48.2% at 10 DAP to 23.9% at 22 DAP. The raffinose content increased significantly, the fructose content kept decreasing, the contents of stachyose and glucose significantly increased firstly and then decreased during seed development. The relative expression levels of 64SHsp18.0 and Os03g0267200 genes increased significantly from 10 to 19 DAP, and then decreased. The moisture content, the contents of raffi-nose, stachyose and sucrose, thousand seed weight of fresh seeds and dry seeds, relative transcriptions of 64SHsp18.0 and Os03g0267200 genes were all significantly correlated with seed germination during seed maturity.%系统研究了不同成熟期Y两优689杂交水稻种子的活力、可溶性寡糖含量和小分子热激蛋白表达量的变化.运用模糊隶属函数法综合分析种子的发芽率、发芽势、发芽指数、活力指数、平均发芽时间、根数、根长、苗高、苗重发现,授粉后19~34 d的种子生活力与活力均较高.种子成熟过程中含水量呈下降趋势,授粉后10~22 d,种子含水量从48.2%下降至23.9%;鲜种子棉子糖与果糖含量分别呈上升和下降趋势;水苏糖和葡萄糖呈先上升后下降趋势.授粉后10~19 d,小分子热激蛋白64SHsp18.0和Os03g0267200基因的表达量均呈上升趋势,此后相对表达量下降.水分、棉子糖、水苏糖和蔗糖等物质含量,鲜种子及干种子的千粒重,以及64SHsp18.0和Os03g0267200基因的相对表达量均与种子发芽密切相关.