首页> 外文期刊>Molecular Microbiology >Resting complexes of the persistent yeast 20S RNA Narnavirus consist solely of the 20S RNA viral genome and its RNA polymerase p91.
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Resting complexes of the persistent yeast 20S RNA Narnavirus consist solely of the 20S RNA viral genome and its RNA polymerase p91.

机译:持久性酵母20S RNA鼻病毒的静止复合体仅由20S RNA病毒基因组及其RNA聚合酶p91组成。

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The positive strand 20S RNA narnavirus persistently infects Saccharomyces cerevisiae. The 20S RNA genome has a single gene that encodes the RNA-dependent RNA polymerase (p91). 20S RNA forms ribonucleoprotein resting complexes (RNPs) with p91 and resides in the cytoplasm. Here we found no host proteins stoichiometrically associated with the RNP by pull-down experiments. Furthermore, 20S RNA, when expressed from a vector in Escherichia coli, formed RNPs with p91 in the absence of yeast proteins. This interaction required the 3' cis signal for complex formation. Moreover, when 23S RNA, the genome of another narnavirus, was expressed in E. coli, it also formed RNPs with its RNA polymerase p104. Finally, when both RNAs were expressed in the same E. coli cell, they formed RNPs only with their cognate RNA polymerases. These results altogether indicate that narnaviruses RNPs consist of only the viral genomes and their cognate RNA polymerases. Because the copy number of the RNPs can be induced almost equivalent to those of rRNAs in some yeast strains, the absence of host proteins may alleviate the burden on the host by not sequestering proteins into the RNPs. It may also contribute to the persistent infection of narnaviruses by decreasing their visibility.
机译:正链20S RNA纳纳病毒持续感染酿酒酵母。 20S RNA基因组具有一个编码RNA依赖性RNA聚合酶(p91)的单一基因。 20S RNA与p91形成核糖核蛋白静止复合体(RNP),并位于细胞质中。在这里,我们没有通过下拉实验发现与RNP在化学计量上相关的宿主蛋白。此外,当从载体在大肠杆菌中表达时,20S RNA在没有酵母蛋白的情况下与p91形成RNP。这种相互作用需要3'顺式信号来形成复合物。此外,当另一种成年病毒的基因组23S RNA在大肠杆菌中表达时,它也通过其RNA聚合酶p104形成了RNP。最后,当两个RNA在同一个大肠杆菌细胞中表达时,它们仅与它们的同源RNA聚合酶形成RNP。这些结果完全表明,纳纳病毒的RNP仅由病毒基因组及其同源RNA聚合酶组成。因为在某些酵母菌株中可以诱导RNP的拷贝数几乎等同于rRNA的拷贝数,所以不存在宿主蛋白可以通过不将蛋白螯合到RNP中来减轻宿主的负担。它也可能通过降低它们的可见性来促进纳纳病毒的持续感染。

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