STUDY ON THE EFFECT OF 3,5,4 '-TRIMETHOXY-TRANS-STILBENE ON THE REGULATION OF GASTRIC CANCER CELL APOPTOSIS PATHWAY AND ITS SENSITISING EFFECT ON CISPLATIN CHEMOTHERAPY

摘要

The purpose of the research was aimed to explore the inhibition effect of 3,5,4'-trimethoxy-trans-stilbene (BTM) on human gastric cancer cells and its mechanism. The results indicated the higher the concentration of trimethoxystilbene was, the higher the inhibition rates on MKN-45 and MGC-803 cell lines were, with a significant dose-depending relationship. The half maximum inhibitory concentration of cisplatin on MKN-45 and MGC-803 cell lines treated with 20 mu mol/l trimethoxystilbene were significantly lower than that of the control group (0 mol/l), showing that the cisplatin IC50 of MGC-803 cell lines could be reduced by trimethoxystilbene. In addition, the combination of trimethoxystilbene and cisplatin can obviously induce apoptosis of MKN-45 and MGC-803 cells, which is much higher than that of the single use of the two. The higher the concentration of trimethoxystilbene was, the higher the apoptosis rates of MKN-45 and MGC-803 cells were, there was a significant dose-effect relationship. And with the increased concentration of the drug, the proportion of G0/G1 was increased in a certain concentration effect relationship, suggesting that the G2/M phase of cisplatin resistant gastric cancer cell was blocked by trimethoxystilbene. Besides, trimethoxystilbene combined with cisplatin significantly inhibited the expression of anti-apoptotic protein Bcl-2, and increased the expression of Pro apoptotic protein Bax.