Boswellic acid sensitizes gastric cancer cells to Cisplatin-induced apoptosis via p53-mediated pathway

摘要

CDDP had differential effect on proteins expression and induced apoptosis in GC cells. a Western blot shows the effect of CDDP on protein expression in AGS and NCI - N87 cell lines. The expression of tumor suppressor protein 53 increased with CDDP concentration, whilst Akt and NFkB expressions reduced, notice the cleaved PARP which indicates apoptosis b Graphical presentation of apoptosis percentages in AGS and NCI-N87 cell lines treated with different concentrations of CDDP showing significant increase in apoptosis in comparison to control concentration (*P < 0.05, **P < 0.01, ***P < 0.001). The response of AGS cells is more evident than NCI-N87. c Flow cytometry graphs showing the effect of different doses of CDDP on AGS and NCI-N87 cells, each graph shows alive cells, early and late apoptotic cells, and dead cells