Objective To study the regulating effects of 3,5,4' - trimethoxystilbene(BTM)on the TNF - α production and the NF - κB activation in murine macrophages activated by LPS. Methods The murine macrophage cell line RAW264. 7 cells were cultured and dif-ferent concentrations of BTM or resveratrol were added,then LPS activated cells were added. The cellular supernatant was collected for detecting the TNF - α activity by the L929 cell crystal violet staining and the cell climbing slides were prepared for detecting the NF - κB expression in macrophages by immunocytochemistry method. Results BTM and resveratrol had no cytotoxicity to L929 cells. After processing by different concentrations of BTM and resveratrol,the TNF - α production level and the positive rate of NF - κB expression in macrophages showed the dose - dependent decrease. The ability of BTM for inhibiting the TNF - α production level and NF - κB expressing in macrophages was greater than that of resveratrol. The activity of macrophages for producing TNF - α and the pos-itive cell rate of NF - κB were negatively correlated with the drug concentration,while the TNF - α activity was positively correlated with the positive cell rate of NF - κB. Conclusion BTM and resveratrol in the concentration range of 5 - 80 μmol / L inhibit TNF - αproduction with a concentration dependent manner by inhibiting NF - κB activation. The ability of BTM for inhibiting TNF - α production and the NF - κB activation in macrophages is greater than that of resveratrol.%目的：研究三甲氧基二苯乙烯（BTM）对小鼠巨噬细胞经脂多糖(LPS)诱导后产生肿瘤坏死因子-α(TNF -α)及细胞核因子-κB (NF -κB)活化的调控作用。方法培养 RAW246.7小鼠巨噬细胞，加入不同浓度的 BTM 或白藜芦醇，再加入 LPS 活化细胞，收集细胞上清液用 L929细胞结晶紫染色法检测 TNF -α的活性，制作细胞爬片用免疫细胞化学法检测巨噬细胞中 NF -κB 的表达。结果 BTM 和白藜芦醇本身对 L929细胞无细胞毒性；经不同浓度 BTM 和白藜芦醇处理后巨噬细胞产生 TNF -α的水平及表达 NF -κB 的阳性率呈剂量依赖性减少，BTM 抑制巨噬细胞产生 TNF -α及表达 NF -κB 的能力大于白藜芦醇，巨噬细胞产生 TNF -α的活性及 NF -κB 的阳性细胞率均与药物浓度呈负相关，TNF -α的活性和 NF -κB 的阳性细胞率呈正相关。结论 BTM 和白藜芦醇在浓度为5~80μmol / L 范围内，呈浓度依赖性地通过抑制 NF -κB 的活化来抑制 TNF -α的产生，且 BTM 抑制巨噬细胞产生 TNF -α和 NF -κB 活化的能力大于白藜芦醇。
展开▼
