Ginseng saponins Rg1's effect on the expression of tumor necrosis factor alpha, monocyte chemotactic factor protein-1 in diabetic nephropathy rats

摘要

abstract_textpDiabetic nephropathy (DN) is a common complication of diabetes mellitus. Inflammation mechanism is a key factor for DN sustainable development. Monocyte chemotactic protein 1 (MCP-1) is a main factor of DN inflammatory infiltration, which generates a large amount of tumor necrosis factor alpha (TNF-alpha). Ginsenoside Rg1 can reduce 24 h urine protein and blood beta 2 microglobulin levels. However, the role of ginsenoside Rg1 in intervening inflammation and improving renal function in DN is still unclear. This study explored the impact of ginsenoside Rg1 on TNF-alpha and MCP-1 expression in diabetic nephropathy rat. 80 male rats were equally randomly divided into normal control, diabetes model group, ginsenoside Rg1 group, and irbesartan (ARB) group. Diabetes model was established by streptozotocin (STZ) abdominal injection at 65 mg/kg. Blood glucose, 24 h urinary protein, and serum creatinine level were detected. MCP-1 and serum TNF-alpha levels were tested by ELISA. MCP-1 and TNF-alpha mRNA expression were determined by Real time PCR. Compared with model group, 24 h urine protein and serum creatinine levels significantly reduced in treatment group (P 0.05). Ginsenoside Rg1 group and ARB group presented declined MCP-1 and TNF-a levels than model group (P 0.01). Ginsenoside Rg1 can improve renal function in DN, which facilitates renal pathological damage repair and renal protection./p/abstract_text