Recombinant phagemid DNA PHEN-TAB, containing the gene of single chain human antibodies are capable of binding tumor necrosis factor alpha RIGHTS; Recombinant strains of bacteria ESCHERICHIA COLI-single-chain antibody PRODUCER AGAINST HUMAN SCTAB tumor necrosis factor alpha RIGHTS; Recombinant soluble single-chain antibody AGAINST HUMAN SCTAB tumor necrosis factor alpha RIGHTS

摘要

1. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u0444u0430u0433u043cu0438u0434u043du0430u00a0 dna pHEN - tab, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 gene u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e human antibodies capable of u0441u0432u00a0u0437u044bu0432u0430u0442u044c tumor necrosis factor alpha alpha rights of 5237 sukhoy. and u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du043eu0439 weighs 3.46 - u0432u043au043bu044eu0447u0430u044eu0449u0430u00a0:;SfiI - NotI - vector fragment u0444u0430u0433u043cu0438u0434u044b pHEN2 (mrc, u0432u0435u043bu0438u043au043eu0431u0440u0438u0442u0430u043du0438u00a0) the size of the 4495 sukhoy, containing the site of initiation of replication of plasmid pUC18, site of initiation of replication u043du0438u0442u0447u0430u0442u043eu0433u043e bacteriophage m13, consistency, for u0433u0438u0441u0442u0438u0434u0438u043du043eu0432u044bu0439 u0433u0435u043au0441u043eu043cu0435u0440 and epitope with myc, promoter lac - u043eu043fu0435u0440u043eu043du0430 e coli, the gene - lactamase (bla), u0441u0443u043fu0440u0435u0441u0441u0438u0440u0443u0435u043cu044bu0439 stop codon TAG, u0444u0440u0430u0433u043c kent gene protein pIII u0444u0430u0433u0430 m 13;;SfiI - NotI - fragment of 742 sukhoy.a synthetic gene, u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0449u0438u0439 u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e human antibodies capable of u0441u0432u00a0u0437u044bu0432u0430u0442u044c tnf - rights, which u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu0439 domain u0442u00a0u0436u0435u043bu043eu0439 chain u0441u043eu0435u0434u0438u043d yong with u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu043c light chain immunoglobulin domain of human dna sequences, u043au043eu0434u0438u0440u0443u044eu0449u0435u0439 flexible u043fu0435u043fu0442u0438u0434u043du044bu0439 linker ser (Gly4Ser) 2AlaArgGlySerGly4Ser with n u0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e sequence contained in u0444u0438u0433.2;;contains:;promoter lac - u043eu043fu0435u0440u043eu043du0430 e. coli;;genetic marker, gene - lactamase (bla), u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0449u0438u0439 sustainability u0442u0440u0430u043du0441u0444u043eu0440u043cu0438u0440u043eu0432u0430u043du043du044bu0445 u0444u0430u0433u043cu0438u0434u043eu0439 pHEN - tab cells of e. coli to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443;;unique restriction sites u0443u0437u043du0430u0432u0430u043du0438u00a0 u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0430u043cu0438 with the following coordinates: HindIII - 235, SfiI - 328, NotI - 1070, EcoRI - 2361.;2. a recombinant strain of bacteria escherichia coli HB2151 / pHEN - tab containing the recombinant dna u0444u0430u0433u043cu0438u0434u043du0443u044e pHEN - tab, under paragraph 1, u0434u0435u043fu043eu043du0438u0440u043eu0432u0430u043du043du044bu0439 in enterprise of u043au043au043c u0433u043du0446 wb "vector" nome rum in 1077, a producer u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e human antibodies capable of u0441u0432u00a0u0437u044bu0432u0430u0442u044c tumor necrosis factor alpha alpha.;3. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu0435 u0440u0430u0441u0442u0432u043eu0440u0438u043cu043eu0435 u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0435 antibody rights scTAB capable of u0441u0432u00a0u0437u044bu0432u0430u0442u044c tumor necrosis factor alpha alpha rights obtained according to paragraph 2, with amin u043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence contained in u0444u0438u0433.2.