Agrobacterium-mediated transformation of plants: Transfer of vector DNA fragments in the plant genome

摘要

The review presents experimental data on the integration in the plant nuclear genome of vector sequences during Agrobacterium-mediated transformation. Main attention is given to the frequency and mechanisms of this event among transgenic plants. The factors influencing the frequency of this event, as well as the ways of developing special constructs for elimination of vector backbone sequences as undesirable from the point of view of commercial use of genetically modified plants, are considered.