Utilization of aromatic amino acids as nitrogen sources inBrevibacterium linens: an inducible aromatic amino acid aminotransferase

摘要

The first step of the utilization of the aromatic amino acids as sole nitrogen sources byBrevibacterium linensstrain 47 was found to be a transamination. The deaminated metabolites of the amino acids were detected in culture supernatants, and the enzyme activity was identified in cell free extracts. The cells contained increased aromatic amino acid aminotransferase activities on growth on the aromatic amino acids as sole nitrogen sources. Two aromatic aminotransferases (AT-I and AT-II) were separated upon diethylaminoethyl-Trisacryl M column chromatography of cell free extracts. Only AT-I was responsible for the increased level of aromatic amino acid aminotransferase activity of ‘induced’ cells. The results suggested a catabolic role of AT-I in v