College of Pharmacy,Chungnam National University;Daejeon 305-764,Korea:College of Pharmacy,Catholic University of Daegu;Gyeongsan 712-702,Korea;Korea Food and Drug Administration;Seoul 122-704,Korea.

摘要

A homology-based cloning strategy yielded a cDNA clone,designated Sd-cam,encoding calmodulin protein from Scoparia dulcis.The restriction digests of genomic DNA of S.dulcis showed a single hybridized signal when probed with the fragment of this gene in Southern blot analyses,suggesting that Sd-cam occurs as a sole gene encoding calmodulin in the plant.The reverse-transcription polymerase chain reaction analysis revealed that Sd-cam was appreciably expressed in leaf,root and stem tissues.It appeared that transcription of this gene increased transiently when the leaf cultures of S.dulcis were treated with methyl jasmonate and calcium ionophore A23187.These results suggest that transcriptional activation of Sd-cam is one of the early cellular events of the methyl jasmonate-induced responses of S.dulcis.