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首页> 外文期刊>Lipids >AN IMPROVED METHOD FOR RAPID ANALYSIS OF THE FATTY ACIDS OF GLYCEROLIPIDS
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AN IMPROVED METHOD FOR RAPID ANALYSIS OF THE FATTY ACIDS OF GLYCEROLIPIDS

机译:快速分析甘油脂中脂肪酸的改进方法

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摘要

An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH-catalyzed transesterification and high-speed gas chromatography. Glycerolipids (20-40 mg) were mixed with 2 mt of hexane and 0.2 mt of 2 M methanolic KOH at room temperature for 1-2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP-2340 at 240 degrees C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed within 5 min for common vegetable oils and 8 min for fish oils. [References: 13]
机译:描述了一种确定甘油脂的脂肪酸组成的改进的快速程序。该过程包括KOH催化的酯交换反应和高速气相色谱。在室温下将甘油脂(20-40 mg)与2公吨己烷和0.2公吨2 M甲醇KOH混合1-2分钟。通过气相色谱在240℃下于10%SP-2340上分析己烷层中的脂肪酸甲酯。亚油酸甲酯和二十二碳六烯酸甲酯分别在2分钟和5分钟内洗脱。普通植物油的分析在5分钟内完成,鱼油的分析在8分钟内完成。 [参考:13]

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