沙棘油脂肪酸、sn-2位脂肪酸及甘三酯的测定与分析

摘要

采用超临界CO2萃取得到沙棘籽油和沙棘全果油,并采用气相色谱和超高效液相色谱-飞行时间质谱对其脂肪酸、sn-2位脂肪酸及甘三酯组成进行测定与分析.结果表明:沙棘籽油中亚油酸、亚麻酸和油酸的含量较多,分别为38.71％、25.66％和20.68％,这3种脂肪酸也是其主要的sn-2位脂肪酸,含量分别为46.46％、25.49％和23.02％;LOL(13.65％)、OLO(12.06％)和LLnO(11.23％)是沙棘籽油的主要甘三酯类型;沙棘全果油中棕榈油酸(36.86％)、棕榈酸(27.27％)和油酸(18.00％)的含量较高,主要的sn-2位脂肪酸为棕榈油酸(45.57％)和油酸(32.06％);沙棘全果油的主要甘三酯为POPo(14.38％)、PoOPo(13.77％)和OPO(12.06％).%Two kinds of sea buckthorn oils were individually extracted from the sea buckthorn pulp and seed by supercritical CO2 technique.Their fatty acid,sn-2 fatty acid and triacylglycerol compositions were determined by GC and UPLC-Q-TOF/MS,respectively.The results demonstrated that linoleic acid (38.71％),linolenic acid (25.66％)and oleic acid (20.68％)were the major fatty acids in the seed oil.Meanwhile,their contents in sn-2 fatty acids were also dominant,reaching 46.46％,25.49％ and 23.02％,respectively.The main triacylglycerols in the seed oil were LOL (13.65％),OLO (12.06％)and LLnO (11.23％).Palmitoleic acid (36.86％),palmitic acid (27.27％)and oleic acid (18.00％)were the predominant fatty acids in the pulp oil.Palmitoleic acid (45.57％)and oleic acid (32.06％) were the main sn-2 fatty acids,moreover,POPo (14.38％),PoOPo (13.77％)and OPO (12.06％) were the predominant triacylglycerols in the pulp oil.