首页> 外文期刊>Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis >Absolute number of circulating CD34+ cells is abnormally low in refractory anemias and extremely high in RAEB and RAEB-t; novel pathologic features of myelodysplastic syndromes identified by highly sensitive flow cytometry.
【24h】

Absolute number of circulating CD34+ cells is abnormally low in refractory anemias and extremely high in RAEB and RAEB-t; novel pathologic features of myelodysplastic syndromes identified by highly sensitive flow cytometry.

机译:在难治性贫血中,循环中的CD34 +细胞的绝对数量异常低,而RAEB和RAEB-t则绝对高。高灵敏流式细胞仪鉴定骨髓增生异常综合征的新病理特征。

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

We scored absolute numbers of circulating CD34+ cells by a highly sensitive triple-color flow cytometric analysis using CD45 monoclonal antibody, CD34 monoclonal antibody and propidium iodide. Forty-one patients with MDS (RA: 27, RARS: 1, RAEB: 6, RAEB-t: 3,CMML: 4), 12 patients with aplastic anemia (AA) and 36 age-adjusted normal subjects were studied. RA had significantly decreased numbers of cells expressing CD34 (0.21 +/- 0.29 x 10(6)/l) compared with normal subjects (0.81 +/- 0.36 x 10(6)/l)(P < 0.001). This low number of CD34+ cells in RA resembles the case of AA (0.39 +/- 0.73 x 10(6)/l). In light-scatter analysis, the CD34+ cells of RA patients were distributed mainly in low forward scatter (FSC) (lymphocyte region). In contrast, the CD34+ cell counts were extremely high in patients with RAEB (46.54 +/- 71.37 x 10(6)/l) and RAEB-t (57.00 +/- 52.36 x 10(6)/l) (P < 0.001) and the CD34+ cells were observed in high FSC (blast region).CMML patients showed moderately increased numbers of CD34+ cells (3.69 +/- 4.64 x 10(6)/l). Thus, there was a distinct difference in cell size and number of circulating CD34+ cells between RA and RAEB/RAEB-t. In univariate and multivariate analysis, a high CD34+ cell count (> or = 1.0 x 10(6)/l) was a poor prognostic factor. This method allows one to distinguish RA from other MDS subtypes more reliably than by morphology alone and provides early signs of progression to acute leukemia.
机译:我们通过使用CD45单克隆抗体,CD34单克隆抗体和碘化丙啶的高度敏感的三色流式细胞术分析,对循环中CD34 +细胞的绝对数量进行了评分。研究了41例MDS患者(RA:27,RARS:1,RAEB:6,RAEB-t:3,CMML:4),12例再生障碍性贫血(AA)患者和36位年龄校正后的正常受试者。与正常受试者(0.81 +/- 0.36 x 10(6)/ l)相比,RA的CD34表达细胞数明显减少(0.21 +/- 0.29 x 10(6)/ l)(P <0.001)。 RA中CD34 +细胞的数量很少,类似于AA(0.39 +/- 0.73 x 10(6)/ l)。在光散射分析中,RA患者的CD34 +细胞主要分布在低前向散射(FSC)(淋巴细胞区域)中。相反,RAEB(46.54 +/- 71.37 x 10(6)/ l)和RAEB-t(57.00 +/- 52.36 x 10(6)/ l)患者的CD34 +细胞计数极高(P <0.001 ),并且在高FSC(爆炸区域)中观察到CD34 +细胞.CMML患者显示CD34 +细胞数量适度增加(3.69 +/- 4.64 x 10(6)/ l)。因此,RA和RAEB / RAEB-t之间的细胞大小和循环CD34 +细胞数量存在明显差异。在单变量和多变量分析中,高CD34 +细胞计数(>或= 1.0 x 10(6)/ l)是不良的预后因素。这种方法使人们能够比仅通过形态学更可靠地将RA与其他MDS亚型区分开,并提供了发展为急性白血病的早期迹象。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号