A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples

摘要

Using micro high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS) a simple and fast method for the quantitative determination of 26 mycotoxins was developed. Sample preparation consists of a single extraction step and a dilute-and-shoot approach without further cleanup. With a total run time of 9 min and solvent consumption below 0.3 mL per chromatographic run, the presented method is cost-effective. All toxins regulated by the European Commission with maximum or guidance levels in grain products (fumonisins B_1 and B_2 (FB_1 and FB_2)); deoxynivalenol (DON); aflatoxins B_1 G_1, B_2, and G_2 (AFB_1, AFG_1, AFB_2, and AFG_2); ochratoxin A (OTA); T-2 and HT-2 toxins; and zearalenone (ZEN) can be quantified with this method. Furthermore, the enniatins B, Bl, A, and Al (EnB, EnBl, EnA, and EnA1); beauvericin (BEA); 3-acetyl-deoxynivalenol (3-AcDON); fusarin C (FusC); sterigmatocystin (STC); gliotoxin (GT); and the Alternaria toxins altemariol (AOH), alternariol monomethyl ether