Development of analytical methods of spinosad in agricultural commodities by HPLC with UV detector and monitoring.

摘要

The purpose of this study was to develop a methodology to detect spinosad which is difficult to analyse in simultaneous analysis of pesticide residues. Spinosad was analysed using HPLC with UV detection at 250 nm. Correlation coeff. (r2) for standard curve of spinosad A and D at a standard concn. of 0.1-5.0 mg/kg were 0.999. Limit of quantitation of HPLC analysis was 0.005 mg/kg while limit of detection was 0.001 mg/kg. Recovery experiments were conducted with 5 representative agricultural products to validate the analytical method. Recovery ranged from 74.9% to 104.0% and relative s.d. were <10%. Spinosad residues were investigated in 16 commodities collected from 22 provinces of Korea. No residues were detected in any of the samples.