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Validation of an HPLC Analytical Method for Determination of Biogenic Amines in Agricultural Products and Monitoring of Biogenic Amines in Korean Fermented Agricultural Products

机译:HPLC分析方法测定农产品中的生物胺并监测韩国发酵农产品中的生物胺的有效性

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摘要

An HPLC analytical method was validated for the quantitative determination of biogenic amines in agricultural products. Four agricultural foods, including apple juice, Juk, corn oil and peanut butter, were selected as food matrices based on their water and fat contents (i.e., non-fatty liquid, non-fatty solid, fatty liquid and fatty solid, respectively). The precision, accuracy, recovery, limit of detection (LOD) and quantification (LOQ) were determined to test the validity of an HPLC procedure for the determination of biogenic amines, including tryptamine, β-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, in each matrix. The LODs and LOQs for the biogenic amines were within the range of 0.01~0.10 mg/kg and 0.02~0.31 mg/kg, respectively. The relative standard deviation (RSD) of intraday for biogenic amine concentrations ranged from 1.86 to 5.95%, whereas the RSD of interday ranged from 2.08 to 5.96%. Of the matrices spiked with biogenic amines, corn oil with tyramine and Juk with putrescine exhibited the least accuracy of 84.85% and recovery rate of 89.63%, respectively, at the lowest concentration (10 mg/kg). Therefore, the validation results fulfilled AOAC criteria and recommendations. Subsequently, the method was applied to the analysis of biogenic amines in fermented agricultural products for a total dietary survey in Korea. Although the results revealed that Korean traditional soy sauce and Doenjang contained relatively high levels of histamine, the amounts are of no concern if these fermented agricultural products serve as condiments.
机译:HPLC分析方法经验证可用于定量测定农产品中的生物胺。根据它们的水和脂肪含量(分别为非脂肪液体,非脂肪固体,脂肪液体和脂肪固体),选择了四种农业食品,包括苹果汁,菊糖,玉米油和花生酱作为食品基质。确定了精密度,准确性,回收率,检测限(LOD)和定量(LOQ),以测试HPLC程序测定生物胺的有效性,这些生物胺包括色胺,β-苯乙胺,腐胺,尸胺,组胺,酪胺,每个基质中都有亚精胺和亚精胺。生物胺的LOD和LOQ分别在0.01〜0.10 mg / kg和0.02〜0.31 mg / kg的范围内。生物胺浓度的日内相对标准偏差(RSD)为1.86至5.95%,而日间相对标准偏差为2.08至5.96%。在掺有生物胺的基质中,最低浓度(10 mg / kg)的玉米油,酪胺和Juk和腐胺的准确度最低,分别为84.85%和89.63%。因此,验证结果符合AOAC标准和建议。随后,该方法被用于分析发酵农产品中的生物胺,在韩国进行了总膳食调查。尽管结果表明韩国传统酱油和大酱中的组胺含量相对较高,但如果这些发酵的农产品用作调味品,其含量并不重要。

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