Development of Predictive Growth Model of Imitation Crab Sticks Putrefactive Bacteria Using Mathematical Quantitative Assessment Model

摘要

Predictive growth model of putrefactive bacteria of surimi-based imitation crab in the modified surimi-based imitation crab (MIC) broth was investigated. The growth curves of putrefactive bacteria were obtained by measuring cell number in MIC broth under different conditions (Initial cell number, 1.0 x 10~2, 1.0 x 10~3 and 1.0 x 10~4 colony forming unit (CFU)/mL; temperature, 15°C, 20°C and 25°C) and applied them to Gompertz model. The microbial growth indicators, maximum specific growth rate constant (k), lag time (LT) and generation time (GT), were calculated from Gompertz model. Maximum specific growth rate (k) of putrefactive bacteria was become fast with rising temperature and fastest at 25°C. LT and GT were become short with rising temperature and shortest at 25°C. There were not significant differences in k, LT and GT by initial cell number (p > 0.05). Polynomial model, k = -0.2160 + 0.0241T - 0.0199A_0, and square root model, k = 0.02669 (T-3.5689), were developed to express the combination effects of temperature and initial cell number. The relative coefficient of experimental k and predicted k of polynomial model was 0.87 from response surface model. The relative coefficient of experimental k and predicted k of square root model was0.88. From above results, we found that the growth of putrefactive bacteria was mainly affected by temperature and the square root model was more credible than the polynomial model for the prediction of the growth of putrefactive bacteria.