Studies on cultural characteristics for high density fermentation of Phellinus linteus WI-001

摘要

Various environmental factors such as pH, temperature and initial glucose concentration were investigated for enhancing cell growth in fermentations of Phellinus linteus WI-001, a producer of polysaccarides with potent anticancer activities. Optimal aH and temperature were around 5.5 and 28 deg C, respectively. Relatively little variation of aH was observed ranging between 5.5 and 6.5 during the whole fermentation period. Maximum cell concentration and specific growth rate were investigated in the media containing initial glucose concentrations of 0.5 percent, 1 percent, 2 percent, 3 percent and 4 percent. High initial glucose concentration enhanced biomass production but showed negative effect on specific growth rate. In bioreactor experiments with various feeding strategies, increases of 28 percent and 42 percent in final cell concentration were obtaind as compared to conventional batch process, by adopting pulse and continuous supplement of 2 percent glucose solution, respectively.