Determination of Aspirin and Its Major Metabolites in Human Urine by Gas Chromatography-Mass Spectrometry, II

摘要

A method is described which determines aspirin and its major metabolites in human urine. The samples are specifically treated for the determination of free metabolites (all nonconjugated forms) and total metabolites (free and conjugated forms), followed by extraction through solid phase Cu cartridges. The extraction procedure yields excellent recovery for all metabolites. AH of the metabolites are then derivatized to their respective trimethylsilyl forms, using the standard addition method to enhancequantitation. Using SIM and monitoring the single, high mass ion yields unsurpassable specificity, selectivity, and sensitivity on the GC/MS. The power of gas chromatography coupled with mass spectrometry enables the identification of unknown metabolites, even from the complex urinary matrix.