Method for determination of nucleosides in urine with liquid chromatography-mass spectrometry

摘要

This invention relates to a method of detecting a nucleoside in the urine, based on high performance liquid chromatography (HPLC) more particularly, a method of detecting a nucleoside in the urine of the present invention includes a first step for removing the protein from the urine sample; A second step of the analytical column the sample passed through the protein removal through the first stage; And a specimen passes through the column, mass spectrometry analysis (MS), characterized in that a third step of analyzing using a. ; This invention is a high-performance direct After only simple filtering process without pre-treatment and injected into the liquid chromatography / mass spectrometry, and also ensure a desired assay sensitivity with a small amount of sample, help reduce the extraction operation and the time and money is wasted due to such operation derivatized, may simplify the procedure to the advantage of easy operation . Therefore, when using the present invention, a simultaneous analysis of the obtained sensitivity analysis, it is possible to satisfy the accuracy, reproducibility, ease and speed of analysis and so on. This because it is possible to know exactly the amount of nucleosides in the urine, Alzheimer's disease, as effective biomarkers (biomarker), such as cancer and can provide a proper judgment index.