The invention discloses a kind of all metabolins of extraction to be present in the comprehensive mutual deviation metabolin control group of biology sample detection and test group to verify in meaning. More particularly it relates to include first step, sub-fraction solid phase extraction method is extracted in analysis, the fraction of acquisition, it is different by extracting remaining fraction and liquid-liquid extraction method and two pH value of fraction and liquid-liquid extraction method extraction, after hydrolyzing, use gas chromatography-mass spectrum; It can statistically handle that one second step chromatographic results, which are converted to numerical value,; And third step, analysis numerical value (PCA) are detected with principal component analysis and discriminant analysis (DA) in control group and test group. According to the present invention, the difference between two groups can be with the chromatogram at the peak in complete detection and region, without standard items or the quantitative approach of verifying. Therefore, it is possible to body metabolic alterations be detected, due to the disease or gene mutation, without accurate quantitative analysis metabolin.
展开▼
