METHOD FOR THE ANALYSIS OF METABOLITE DIFFERENCES BETWEEN TWO BIOLOGICAL SAMPLES WITH GAS CHROMATOGRAPHY-MASS SPECTROMETRY

摘要

Provided is a method for analyzing metabolite differences between a control group and an experimental group by extracting all metabolites by a standard extracting method from biological samples, obtaining a chromatogram by chromatography, quantifying for statistic processing and detecting significant differences between two materials by principle component analysis(PCA) and discriminant analysis(DA). The method for analyzing metabolite differences between two biological samples includes extracting metabolites from biological samples by sequentially performing i) solid state extraction, ii) liquid-liquid extraction under weak acid condition, iii) liquid-liquid extraction under weak acid after hydrolysis, and iv) liquid-liquid extraction under basic condition. Also, the method further includes the steps of analyzing the extracted metabolites by chromatography, converting the result of the chromatography into numbers for statistic processing, and statistically verifying the differences between two biological samples using the converted numbers. The weak acid condition is pH 5 to pH 5.5, and the basic condition is pH 13 to 13.5. The hydrolysis is performed using beta-glucuronidase and arylsulfatase.