ROPROPAGATION AND EVALUATION OF FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY OF SALVIA OFFICINALIS L.

摘要

Salvia officinalis L. (garden sage) is a very popular aromatic plant widely used in pharmaceutical and food industries as well as in cosmetics. Leaf extracts of the herb possess antibacterial, antifungal, antiviral, anti-inflammatory, antitumor and hypoglycemic activity. The medicinal efficiency of sage is due to the essential oils, fiavonoids and other polyphenolic compounds contained in the plant. The aim of this study was to develop an efficient method for micropropagation of S. officinalis as well as to evaluate flavonoid content and antioxidant capacity in leaves of the obtained shoots. Nodal segments from in vitro seedlings were used to establish a micropropagation system. The effect of four different cytokinins - BAP, thidiazuron, zeatin or 2-iP combined with the auxin IAA was examined. The maximum shoot proliferation was obtained when explants were cultured on Murashige and Skoog medium supplemented with 0.5 mg/1 BAP and 0.1 mg/1 IAA. Then the shoots were successfully rooted and adapted inex vitro conditions. Flavonoid composition and antiradical properties of leaves of in vitro developed S. officinalis were determined. Six flavonoid aglycones were identified by TLC. Apigenin and its derivatives apigenin 7-methyl ether, scutellarein 6-methyl ether and scutellarein 6,7-dimefhyl ether as well as luteolin and its derivative 6-hydroxyluteolin 6,7,3 '-trimethyl ether were identified. The antioxidant potential of the methanolic extract was assayed by scavenging of DPPH radicals and presented as IC_(50) values (ug/ml). The metanolic extract of in vitro developed S. officinalis showed a significant free radical scavenging activity(IC_(50)= 22.18 ug/ml).