Collision-induced dissociation of dimeric G-quadruplexes of HIV-1 integrase inhibitors and their complexes by tandem-in-time mass spectrometry

摘要

The collision-dissociation behavior of two novel dimeric G-quadruplexes of HIV-1 integrase inhibitors and their non-covalent complex ions with a perylene derivative (Tel03), polyamides (ImImIm beta Dp and PyPyPy beta Dp), was investigated by tandem-in-time electrospray ionization mass spectrometry (ESI-MS). It was found that the dimeric ion loses five ammonium ions one by one at activation amplitude of 10%, so the loss of NH4+ is the predominant fragmentation pathway at Lower collision energy. When the activation amplitude is increased to 16%, the Loss of guanine nucleobases from backbones of the oligonucleotide is the predominant fragmentation pathway. And the stability of the complex ion of the dimeric G-quadruplex and Tel03 is higher than that of ImImIm beta Dp and PyPyPy beta Dp. The results of the MS/MS spectra of the complex ion indicated that Tel03 binding molecules favor the stabilization of the novel G-quadruplex structure.