Optimized Cell Culture Media Vital to Process Development:Work Efficiently to Enhance Established Cell Lines and Base Medium

摘要

Optimization of cell culture parameters is a vital part of biotherapeutic process development, but it is not without its challenges. One such challenge is the selection of a scalable and appropriate cell culture feed that can work in combination withthe established cell line and a given base medium to achieve the desired titer and growth characteristics.The typical approach is to screen multiple commercially available feeds and identify the most appropriate feed with a specific cell line and base medium combination. However, this technique can be tedious and may not work if the base medium or cell lineis altered during process development.