Effect of selenium on increasing free radical scavenging activities of polysaccharide extracts from a Se-enriched mushroom species of the genus Ganoderma.

摘要

Effects of Se were investigated on the free radical scavenging activities of polysaccharides extracted from Ganoderma lucidum. Chemiluminescent analysis indicated that polysaccharide extracts from Se-enriched G. lucidum (Se-GLPs) protected DNA from hydroxyl radical oxidative damage in a dose dependent manner. Moreover, Se-GLPs exhibited higher radical scavenging activities against superoxide and hydroxyl radicals than non-enriched G. lucidum extracts, as suggested by EPR measurements, indicating that Se plays an important role in increasing the antioxidative activities of the polysaccharide extracts. This was confirmed by spin-trapping experiments showing that at the same polysaccharide concn., abilities of all Se-GLPs samples to scavenge hydroxyl radicals increased with increases in Se contents. Additionally, all Se-GLPs samples demonstrated stronger activities against superoxide radicals than against hydroxyl radicals.