Aucubin prevents interleukin-1 beta induced inflammation and cartilage matrix degradation via inhibition of NF-kappa B signaling pathway in rat articular chondrocytes

摘要

Proinflammatory cytokine interleukin-1 beta (1L-1 beta) plays a crucial role in the pathogenesis of Osteoarthritis (OA) by stimulating several mediators contributed to cartilage degradation. Aucubin, a natural compound derived from plants which has been shown to possess diverse biological activities including anti-inflammatory property, may benefit the IL-1 beta stimulated chondrocytes. The present study was aimed to investigate the effects of Aucubin on IL-1 beta stimulated rat chondrocytes. Rat chondrocytes were cultured and pretreated with Aucubin (1, 10, 20, 50 mu M), and then stimulated with or without IL-1 beta (10 ng/ml). Gene and protein expression of MMP-3, MMP-9, MMP-13, cydooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) was determined by real-time PCR and Western blotting respectively. Nitric oxide (NO) production was quantified by Griess reagent Phosphorylation and nuclear translocation of p65 were detected by western blotting and immunofluorescence, respectively. We found thatAucubin significantly reversed the elevated gene and protein expression of MMP-3, MMP-9, MMP-13, iNOS, COX-2 and the production of NO induced by IL-1 beta challenge in rat chondrocytes. Furthermore, Aucubin was able to suppress the IL-1 beta-mediated phosphorylation and nuclear translocation of p65, indicating Aucubin may possibly act via the NF-kappa B signaling pathway. The present study proposes that Aucubin may be a potential therapeutic choice in the treatment of OA due to its anti-inflammatory and chondroprotective features. (C) 2015 Elsevier B.V. All rights reserved.